|
KMID : 0903620040450060330
|
|
Journal of the Korean Society for Horticultural Science
2004 Volume.45 No. 6 p.330 ~ p.335
|
|
|
Introduction of Two Types of Human Ferritin Gene into Lettuce Plants
|
|
Lee Zu-An
Kim Hae-Yeong
Chung Kyu-Hwan
Park Young-Doo
|
|
|
Abstract
|
|
|
|
Human H- and L-chain ferritin genes were introduced into lettuce (Lactuca sativa L.) plants by Agrobcterium-mediated transformation. These two human ferritin genes were cloned into plant binary vectors, pILTAB 357 and pCAMBIA 1302, respectively. After infection with A. tumefaciens LBA4404 containing pILTAB 357 or pCAMBIA 1302, transformed shoots were induced from selection media and regenerated shoots were rooted in rooting media. To confirm the transfer of human H- or L-chain ferritin genes in the genome of lettuce plants, polymerase chain reaction (PCR) was conducted using specific primers designed from the H- and L- chain ferritin genes, respectively. The PCR fragments of 600 or 550 bp were obtained separately from the transformed plants, which are the predicted size of the H- and L- chain ferritin genes, but not from non-transformed plants. Reverse transcription-PCR showed the transcription of transgene from the transgenic plants. Inductively coupled plasma analysis for determination of Fe concentration showed that several transgenic lettuce lines with ferritin genes had higher concentration than non-tranformed ones.
|
|
|
KEYWORD
|
|
|
Agrobcterium-mediated gene transformation, H-chain ferritin gene, L-chain ferritin gene, inductively coupled plasma analysis
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|